My friends and I did an experiment to test if there are starch on a variegated leaf. The experiment needs boiling water, beaker, ethanol, boiling tube, glass rod, white tile and iodine solution.
1. Place the leaf in the dark for sometime so the leaf will not photosynthesize
2. Take the leaf and put in the beaker and fill the beaker with boiling water
3. Leave the leaf in boiling water for a while to kill it
4. Take the leaf out and put it in the boiling tube then fill it with ethanol
5. Use the glass rod and stir the ethanol until the leaf changes colour (the use of the ethanol is to remove the chlorophyll in the leaf)
6. Take the leaf out and then rinse it in water
7. Place the leaf on the white tile and drop some iodine solution on the leaf
After a while, the iodine solution on the green pigment of the leaf turned blue black while the iodine solution on the non green part of the leaf remained yellowish brown. Hence, I can conclude that chlorophyll is needed for photosynthesis as there is no chlorophyll in the non green part of the variegated leaf.
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